"This is our last message to the Syrian authorities; our main expectation is that these operations stop immediately," Davutoglu said in the message, the strongest yet to the neighboring country and once a close ally.
"If the operations do not stop, we have no more messages about the steps that will be taken," he said at the press conference, but did not clarify what he meant specifically.
"We are in contact and have repeatedly repeated our requests and emphasized expectations," he added.
Davutoglu said operations against civilians had intensified since Thursday night.
"In the context of human rights, it cannot be treated as an internal issue," he told reporters. However, he did not answer their question whether he spoke directly with Syrian President Assad today.
Davutoglu visited Damascus last Sunday and spoke with the Syrian president.
Turkish Prime Minister Erdogan, who spoke with US President Barack Obama last Sunday about the violence in Syria, said he hoped Syria would take steps towards reforms within 10 to 15 days.
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